Science
31 AUGUST 2018
VOL 361, ISSUE 6405
· Special Issue
Technologies Transforming Biology
INTRODUCTIONTO SPECIAL ISSUE
Power couple:Science and technology
BY STEVEMAO, VALDA VINSON
SCIENCE31 AUG 2018 : 864-865
REVIEWS
CRISPR-Casguides the future of genetic engineering
BY GAVIN J.KNOTT, JENNIFER A. DOUDNA
SCIENCE31 AUG 2018 : 866-869
Emergingapplications for DNA writers and molecular recorders
BY FAHIMFARZADFARD, TIMOTHY K. LU
SCIENCE31 AUG 2018 : 870-875
Single-particlecryo-EM—How did it get here and where will it go
BY YIFANCHENG
SCIENCE31 AUG 2018 : 876-880
Visualizing anddiscovering cellular structures with super-resolution microscopy
BY YARONM. SIGAL, RUOBO ZHOU, XIAOWEI ZHUANG
SCIENCE31 AUG 2018 : 880-887
THIS WEEKIN SCIENCE
Researchin Science journals.
SCIENCE31 AUG 2018 : 888
EDITORIAL
Revolutionarytechnologies
BY JEREMY BERG
SCIENCE31 AUG 2018 : 827
EDITORS'CHOICE
This week inother journals.
SCIENCE31 AUG 2018 : 889
PRODUCTS& MATERIALS
New Products
SCIENCE31 AUG 2018 : 929
A weekly roundupof information on newly offered instrumentation, apparatus, and laboratorymaterials of potential interest to researchers.
IN BRIEF
News at a glance
SCIENCE31 AUG 2018 : 828-829
A roundup ofweekly science policy and related news.
IN DEPTH
Can a transgenicchestnut restore a forest icon?
BY GABRIEL POPKIN
SCIENCE31 AUG 2018 : 830-831
Researchers seekpermit to release American chestnut engineered to resist a deadly blight.
New pain drugsmay lower overdose and addiction risk
BY ROBERT F. SERVICE
SCIENCE31 AUG 2018 : 831-832
By slowing action or targeting differentreceptors, altered opioids or alternatives aim to sidestep abuse.
Hybridizationmay give some parasites a leg up
BY ELIZABETH PENNISI
SCIENCE31 AUG 2018 : 832-833
Genomic study helps explain howschistosomiasis gained a foothold in Europe.
Amid fears ofidea theft, NIH targets foreign funding links
BY JOCELYN KAISER, DAVID MALAKOFF
SCIENCE31 AUG 2018 : 834
Agency reminds applicants to report allgrant sources, warns against violating confidentiality of peer review.
In dogs, CRISPRfixes a muscular dystrophy
BY JON COHEN
SCIENCE31 AUG 2018 : 835
Treatment repairs gene in beagles byfurther mutating it, but human trials are far off.
Social sciencestudies get a ‘generous’ test
BY KELLYSERVICK
SCIENCE31 AUG 2018 : 836
New replicationeffort aimed to detect effects overstated in the original reports.
FEATURE
The Alzheimer'sgamble
BY JOCELYN KAISER
SCIENCE31 AUG 2018 : 838-841
Can the NationalInstitute on Aging turn a funding windfall into a treatment for the dreadedbrain disease?
WORKING LIFE
The detour thatbecame a shortcut
BY SIDNEY F. GOUVEIA
SCIENCE31 AUG 2018 : 942
LETTERS
Protecting U.S.temporary waterways
BY JONATHANC. MARSHALL, VICENÇ ACUÑA, DANIEL C. ALLEN, NÚRIABONADA, ANDREW J. BOULTON, STEPHANIE M. CARLSON, CLIFFORD N. DAHM, THIBAULTDATRY, CATHERINE LEIGH, PETER NEGUS, JOHN S.RICHARDSON, SERGI SABATER, R. JAN STEVENSON, ALISHA L.STEWARD, RACHEL STUBBINGTON, KLEMENT TOCKNER, ROSS VANDER VORSTE
SCIENCE31 AUG 2018 : 856-857
Privacy andgenetic genealogy data
BY ELLEN M. GREYTAK, DAVID H. KAYE, BRUCEBUDOWLE, CECE MOORE, STEVEN L. ARMENTROUT
SCIENCE31 AUG 2018 : 857
Chile's salmonescape demands action
BY DANIELGOMEZ-UCHIDA, MARITZA SEPÚLVEDA, BILLY ERNST, TAMARA A.CONTADOR, SERGIO NEIRA, CHRIS HARROD
SCIENCE31 AUG 2018 : 857-858
BOOKS ETAL.
A presidentamplifies unlikely activists
BY LOUISEFABIANI
SCIENCE31 AUG 2018 : 854
In dispatchesfrom the front lines of global warming, Mary Robinson pushes for humane climatepolicies
Understandingthe double slit
BY MÉLANIE FRAPPIER
SCIENCE31 AUG 2018 : 855
Glimpses of quantum truth appear indiverse interpretations of the famous physics experiment
The Book of Why:The New Science of Cause and Effect
SCIENCE31 AUG 2018 : 855
POLICY FORUM
Global carbonintensity of crude oil production
BY MOHAMMAD S.MASNADI, HASSAN M. EL-HOUJEIRI, DOMINIK SCHUNACK, YUNPOLI, JACOB G. ENGLANDER, ALHASSAN BADAHDAH, JEAN-CHRISTOPHEMONFORT, JAMES E. ANDERSON, TIMOTHY J. WALLINGTON, JOULE A.BERGERSON, DEBORAH GORDON, JONATHAN KOOMEY, STEVENPRZESMITZKI, INÊS L. AZEVEDO, XIAOTAO T. BI, JAMES E.DUFFY, GARVIN A. HEATH, GREGORY A. KEOLEIAN, CHRISTOPHEMCGLADE, D. NATHAN MEEHAN, SONIA YEH, FENGQI YOU, MICHAELWANG, ADAM R. BRANDT
SCIENCE31 AUG 2018 : 851-853
New data enabletargeted policy to lessen GHG emissions
PERSPECTIVES
Venoms to therescue
BY MANDËHOLFORD, MARYMEGAN DALY, GLENN F. KING, RAYMOND S. NORTON
SCIENCE31 AUG 2018 : 842-844
Insights intothe evolutionary biology of venoms are leading to therapeutic advances
From oncogenicmutation to dynamic code
BY WALTER KOLCH, CHRISTINA KIEL
SCIENCE31 AUG 2018 : 844-845
Oncogenic BRAF mutations can distortdownstream signaling outcomes
Insect threatsto food security
BY MARKUS RIEGLER
SCIENCE31 AUG 2018 : 846
Pest damage to crops will increasesubstantially in many regions as the planet continues to warm
The reemergenceof yellow fever
BY ALAN D. T. BARRETT
SCIENCE31 AUG 2018 : 847-848
Since 2016, yellow fever outbreaks havebecome a major public health concern
Fusion oncogenes—geneticmusical chairs
BY MARCIN IMIELINSKI, MARC LADANYI
SCIENCE31 AUG 2018 : 848-849
Ewing sarcoma–driver fusion genes canresult from complex genomic rearrangements
18 electrons andcounting
BY P. B.ARMENTROUT
SCIENCE31 AUG 2018 : 849-850
The bonding rulefor transition metal complexes now extends to alkaline earth octacarbonyls
ASSOCIATIONAFFAIRS
Emergingscientific technologies help defend human rights
BY ANNE Q.HOY
SCIENCE31 AUG 2018 : 859-860
Scientistsleverage advancing tools to gather evidence and expand the capacity of humanrights groups
AAAS annualelection: Preliminary announcement
SCIENCE31 AUG 2018 : 860
Federal researchfunding aims to ease societal challenges
BY ANNE Q.HOY
SCIENCE31 AUG 2018 : 861
A key goal is tospread access to scientific excellence and federal research funding nationwide
RESEARCHARTICLES
Cancer mutationsand targeted drugs can disrupt dynamic signal encoding by the Ras-Erk pathway
BY L. J.BUGAJ, A. J. SABNIS, A. MITCHELL, J. E. GARBARINO, J. E.TOETTCHER, T. G. BIVONA, W. A. LIM
SCIENCE31 AUG 2018
Light-activatedsignaling allows analysis of cell signal processing.
Rearrangementbursts generate canonical gene fusions in bone and soft tissue tumors
BY NATHANIEL D. ANDERSON, RICHARD DE BORJA, MATTHEW D.YOUNG, FABIO FULIGNI, ANDREJ ROSIC, NICOLA D.ROBERTS, SIMON HAJJAR, MEHDI LAYEGHIFARD, ANANOVOKMET, PAUL E. KOWALSKI, MATTHEW ANAKA, SCOTTDAVIDSON, MEHDI ZARREI, BADR ID SAID, L. CHRISTINESCHREINER, REMI MARCHAND, JOSEPH SITTER, NALANGOKGOZ, LEDIA BRUNGA, GARRETT T. GRAHAM, ANTHONYFULLAM, NISCHALAN PILLAY, JEFFREY A. TORETSKY, AKIHIKOYOSHIDA, TATSUHIRO SHIBATA, MARKUS METZLER, GINO R.SOMERS, STEPHEN W. SCHERER, ADRIENNE M. FLANAGAN, PETER J.CAMPBELL, JOSHUA D. SCHIFFMAN, MARY SHAGO, LUDMIL B.ALEXANDROV, JAY S. WUNDER, IRENE L. ANDRULIS, DAVIDMALKIN, SAM BEHJATI, ADAM SHLIEN
SCIENCE31 AUG 2018
The gene fusions driving sarcoma growthoften arise by the formation of dramatic genomic loops that rearrange manygenes.
Developmentalbarcoding of whole mouse via homing CRISPR
BY REZA KALHOR, KIAN KALHOR, LEO MEJIA, KATHLEENLEEPER, AMANDA GRAVELINE, PRASHANT MALI, GEORGE M. CHURCH
SCIENCE31 AUG 2018
Multiple, independent barcoding elementsenable lineage tracing in the whole mouse embryo during brain development andpatterning.
Genomic andepidemiological monitoring of yellow fever virus transmission potential
BY N. R.FARIA, M. U. G. KRAEMER, S. C. HILL, J. GOES DE JESUS, R.S. AGUIAR, F. C. M. IANI, J. XAVIER, J. QUICK, L. DUPLESSIS, S. DELLICOUR, J. THÉZÉ, R. D. O. CARVALHO, G.BAELE, C.-H. WU, P. P. SILVEIRA, M. B. ARRUDA, M. A.PEREIRA, G. C. PEREIRA, J. LOURENÇO, U. OBOLSKI, L.ABADE, T. I. VASYLYEVA, M. GIOVANETTI, D. YI, D. J.WEISS, G. R. W. WINT, F. M. SHEARER, S. FUNK, B.NIKOLAY, V. FONSECA, T. E. R. ADELINO, M. A. A.OLIVEIRA, M. V. F. SILVA, L. SACCHETTO, P. O.FIGUEIREDO, I. M. REZENDE, E. M. MELLO, R. F. C. SAID, D.A. SANTOS, M. L. FERRAZ, M. G. BRITO, L. F. SANTANA, M. T.MENEZES, R. M. BRINDEIRO, A. TANURI, F. C. P. DOSSANTOS, M. S. CUNHA, J. S. NOGUEIRA, I. M. ROCCO, A. C. DACOSTA, S. C. V. KOMNINAKIS, V. AZEVEDO, A. O. CHIEPPE, E.S. M. ARAUJO, M. C. L. MENDONÇA, C. C. DOS SANTOS, C. D. DOS SANTOS, A.M. MARES-GUIA, R. M. R. NOGUEIRA, P. C. SEQUEIRA, R. G.ABREU, M. H. O. GARCIA, A. L. ABREU, O. OKUMOTO, E. G.KROON, C. F. C. DE ALBUQUERQUE, K. LEWANDOWSKI, S. T.PULLAN, M. CARROLL, T. DE OLIVEIRA, E. C. SABINO, R. P.SOUZA, M. A. SUCHARD, P. LEMEY, G. S. TRINDADE, B. P.DRUMOND, A. M. B. FILIPPIS, N. J. LOMAN, S. CAUCHEMEZ, L.C. J. ALCANTARA, O. G. PYBUS
SCIENCE31 AUG 2018 : 894-899
MinION genomicand case data on a recent yellow fever epidemic indicate that most infectionsoccurred during visits to forest regions.
REPORTS
The spatialfootprint of injection wells in a global compilation of induced earthquakesequences
BY THOMASH. W. GOEBEL, EMILY E. BRODSKY
SCIENCE31 AUG 2018 : 899-904
The seismichazard distance from injection wells is dependent on rock type and fault density.
High-performanceperovskite/Cu(In,Ga)Se2 monolithic tandem solarcells
BY QIFENG HAN, YAO-TSUNG HSIEH, LEI MENG, JYH-LIHWU, PENGYU SUN, EN-PING YAO, SHENG-YUNG CHANG, SANG-HOONBAE, TAKUYA KATO, VERONICA BERMUDEZ, YANG YANG
SCIENCE31 AUG 2018 : 904-908
Optimization of the interconnectionregion between cells is crucial for high efficiency.
Directionalcontrol of a processive molecular hopper
BY YUJIA QING, SANDRA A. IONESCU, GÖKÇE SUPULCU, HAGAN BAYLEY
SCIENCE31 AUG 2018 : 908-912
A single cargo molecule can be drivenback and forth on a track within a membrane-embedded protein pore.
Observation ofalkaline earth complexes M(CO)8 (M = Ca,Sr, or Ba) that mimic transition metals
BY XUAN WU, LILI ZHAO, JIAYE JIN, SUDIP PAN, WEILI, XIAOYANG JIN, GUANJUN WANG, MINGFEI ZHOU, GERNOTFRENKING
SCIENCE31 AUG 2018 : 912-916
Calcium, strontium, and barium donate electronsto coordinated carbonyls in a bonding motif reminiscent of transition metals.
Increase in croplosses to insect pests in a warming climate
BY CURTIS A. DEUTSCH, JOSHUA J. TEWKSBURY, MICHELLETIGCHELAAR, DAVID S. BATTISTI, SCOTT C. MERRILL, RAYMOND B.HUEY, ROSAMOND L. NAYLOR
SCIENCE31 AUG 2018 : 916-919
Models of insect population growth andmetabolism in a warming climate predict losses of major food crops to insectpests.
Past and futureglobal transformation of terrestrial ecosystems under climate change
BY CONNOR NOLAN, JONATHAN T. OVERPECK, JUDY R. M.ALLEN, PATRICIA M. ANDERSON, JULIO L. BETANCOURT, HEATHER A.BINNEY, SIMON BREWER, MARK B. BUSH, BRIAN M. CHASE, RACHIDCHEDDADI, MORTEZA DJAMALI, JOHN DODSON, MARY E.EDWARDS, WILLIAM D. GOSLING, SIMON HABERLE, SARA C.HOTCHKISS, BRIAN HUNTLEY, SARAH J. IVORY, A. PETERKERSHAW, SOO-HYUN KIM, CLAUDIO LATORRE, MICHELLELEYDET, ANNE-MARIE LÉZINE, KAM-BIU LIU, YAO LIU, A. V.LOZHKIN, MATT S. MCGLONE, ROBERT A. MARCHANT, ARATAMOMOHARA, PATRICIO I. MORENO, STEFANIE MÜLLER, BETTE L.OTTO-BLIESNER, CAIMING SHEN, JANELLE STEVENSON, HIKARUTAKAHARA, PAVEL E. TARASOV, JOHN TIPTON, ANNIEVINCENS, CHENGYU WENG, QINGHAI XU, ZHUO ZHENG, STEPHEN T.JACKSON
SCIENCE31 AUG 2018 : 920-923
Global vegetation change since the LastGlacial Maximum is used as an indicator of transformation under warmingscenarios.
Three-dimensionalgenome structures of single diploid human cells
BY LONGZHITAN, DONG XING, CHI-HAN CHANG, HENG LI, X. SUNNEY XIE
SCIENCE31 AUG 2018 : 924-928
A single-cellchromatin conformation capture method employs transposon-based whole-genomeamplification to detect chromatin contacts.
TECHNICALCOMMENTS
Comment on“Unexpected reversal of C3 versus C4 grassresponse to elevated CO2during a 20-year fieldexperiment”
BY MING NIE, JUNYU ZOU, XIAO XU, CHAOLIANG, CHANGMING FANG, BO LI
SCIENCE31 AUG 2018
Comment on“Innovative scattering analysis shows that hydrophobic disordered proteins areexpanded in water”
BY ROBERTB. BEST, WENWEI ZHENG, ALESSANDRO BORGIA, KARIN BUHOLZER, MADELEINEB. BORGIA, HAGEN HOFMANN, ANDREA SORANNO, DANIELNETTELS, KLAUS GAST, ALEXANDER GRISHAEV, BENJAMIN SCHULER
SCIENCE31 AUG 2018
Response toComment on “Innovative scattering analysis shows that hydrophobic disorderedproteins are expanded in water”
BY JOSHUAA. RIBACK, MICAYLA A. BOWMAN, ADAM ZMYSLOWSKI, CATHERINE R.KNOVEREK, JOHN JUMPER, EMILY B. KAYE, KARL F.FREED, PATRICIA L. CLARK, TOBIN R. SOSNICK
SCIENCE31 AUG 2018
Comment on“Innovative scattering analysis shows that hydrophobic disordered proteins areexpanded in water”
BY GUSTAVOFUERTES, NICCOLO BANTERLE, KIERSTEN M. RUFF, ARITRACHOWDHURY, ROHIT V. PAPPU, DMITRI I. SVERGUN, EDWARD A. LEMKE
SCIENCE31 AUG 2018